Abstrakt

IN SILICO SEQUENCE ANALYSIS AND PREDICTION OF 3-D STRUCTURE OF SOYBEAN TRYPSIN INHIBITOR (SBTI) BY HOMOLOGY MODELLING

P.G. Pohekar, N.J.Chikhale

Soybean trypsin inhibitor is the enzyme which inactivates the trypsin inhibitor and provides a serum free option when subculturing adherent cells. It acts as an inhibitor only when it is in its native state. The present study deals with the sequence analysis and homology modelling of Soybean trypsin inhibitor. The sequence analysis was performed with respect to secondary structure and antigenecity prediction. The secondary structure prediction reveals the extended strand contributing more to structure, where as the antigenecity prediction confirmed the presence of antigenic determinant. The 3D structure was predicted by using modeller. The resultant structure was evaluated by procheck, errat and verify 3D program showing the compatibility of the residues in the structure predicted.