Abstrakt

In Vitro α-Amylase Inhibitory Activity of the Leaves of Tinosporacordifolia

M. Ismail Shareef, P. Jagan Mohan Reddy, S. M. Gopinath, K. S. Dayananda1, Ajay Mandal, Purushotham K.M

Postprandial hyperglycemia is a prime characteristic of diabetes mellitus and has been a focus in the therapy for diabetes. One of the therapeutic approaches which involve decreasing hyperglycemia aims at inhibiting the enzyme α-amylase. The leaves of T. Cordifolia were studied for the presence of amylase inhibitors. In this study area, herbal remedies are considered convenient for management of Type 2 diabetes with postprandial hyperglycemia due to their traditional acceptability and availability, low costs, lesser side effects. Comparative evaluation of alpha amylase inhibitory activities of selected plants extracts. The extracts obtained were subjected to in vitro alpha amylase inhibitory assay using starch azure as a substrate and porcine pancreatic amylase as the enzyme The fractions obtained by successive fractionation using solvents of varying polarity were studied for the presence of primary and secondary metabolites. The total phenolic content of the different fractions was determined by HPLC and was correlated with their amylase inhibitory potential. Similarly, the protein content of the extracts was also estimated to understand the nature of the inhibitor present. This study shows that the leaves of T. Cordifolia were effective in inhibiting α-amylase, thereby proving to be potential anti-hyperglycemic agents.