Abstrakt

Liquid Chromatographic Method Development for the Estimation of Cefpodoxime Proxetil and Clavulanic Acid in Combined Dosage Form.

Dimal A Shah, Piyush A Chaudhary, Usmangani K Chhalotiya, Sunil L Baldania and Kashyap K Bhatt

Liquid chromatographic method was developed for simultaneous quantitative determination of cefpodoxime proxetil and clavulanic acid in their combined dosage form. The separation was achieved by C18 (250 mm × 4.6 mm id, 5μm) column using methanol: water [60:40 (v/v)] as mobile phase at a flow rate of 1.5 mL min-1 and detection at 225 nm. The retention time for CEF (S epimer) & CEF (R epimer) and CLV were found to be 7 & 8.2 and 1.93 min, respectively. The method was found to be linear in the range of 50-250 μg/ml for CEF and 30-150 μg/ml for CLV. The recovery was in the range of 98.14-99.94 % for CEF and 98.60-99.30 % for CLV. The developed method was statistically validated and found to be simple, precise, reproducible and accurate. The developed and validated method was successfully used for the quantitative analysis of commercially available dosage form.