Abstrakt

Protective Effect of Epigallocatechin Gallate on Fluoride-Induced Oxidative Stress Related Haematotoxicity in Rats

S Miltonprabu and S Thangapandiyan

Fluoride (Fl) is a naturally occurring electro negative compound which is classified as potent toxicant and human carcinogen. Erythrocytes are very expedient model to understand the susceptibility of oxidative stress on membrane induced by different xenobiotics. Fl administration (25mg/kg/BW) significantly increased the percentage of hemolysis, lipid peroxidation markers such as thiobarbituric acid reactive substances (TBARS), conjugated dienes (CD), protein carbonyls contents (PC), and altered haematological parameters, with decreased antioxidant enzymes such as superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPX),glutathione-s-transferase (GST), glutathione reductase (GR) and glucose-6-phosphate dehydrogenase (G6PD). The levels of non-enzymic antioxidants (reduced glutathione, vitamin âÂ?Â?C‘ and vitamin âÂ?Â?E‘) and membrane bound ATPases (Na+/K+-ATPase, Mg2+-ATPase and Ca2+-ATPase) were also decreased in F treated rats. Pre oral administration of EGCG (40mg/k/BW) along with Fl for 28 days significantly reduced the levels of TBARS, CD and PC with significantly increased membrane ATPases, membrane integrity, viability, enzymatic and non-enzymatic antioxidants in the RBC‘s of Fl treated rats. In conclusion, the results clearly indicate that EGCG significantly attenuated the Fl induced haematotoxicity in rats.