Kabita Banik, Simanchala Rath and B.K Gupta
A new method for the determination of paraben by High-Performance Liquid Chromatography (HPLC) and determination of Neolone 950 by High- Performance Thin Layer Chromatography (HPTLC) was developed. Skin creams usually consist of many components, including preservatives. Consumer health and safety is the main reason for including preservatives as antimicrobial additives in skin cream formulations. Preservatives are often used as a multi-component mixture to increase the range of microorganisms against which the product is protected. Ingredients in cosmetic products are labelled in accordance with (European Union) EU legislation. Here we established a quantitative method to estimate the concentration of preservative in Herbal skin cream. Their methods were based on High- Performance Liquid Chromatography (HPLC) analysis and performed under different chromatographic conditions. The proposed method was successfully applied to the assay of methylparaben, propyl paraben and neolone 950 in cosmetic products with minimal sample preparation
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