Abstrakt

The Effect of Matrix Metalloproteinase Inhibition on the Shoulder Joint in Diabetic Rats

Esra Circi

Objective: The aim of this study was to reveal the effect of doxycycline related inhibition of the matrix metalloproteinase (MMPs) on the shoulder joint of the diabetic rats. Material and methods: 36 adult Sprague-Dawley male rats were used. To introduce a diabetes, 35 mg/kg intra-peritoneally streptozotocin were applied 24 rats. Control group rats (n=12) received 35 mg/kg an intraperitoneal injection of serum physiologic. 72 h later, doxycycline was applied to diabetic group rats (n=12) and control groups rats (n=6) via orogastric tube 130 mg/ kg per day during the two weeks. Diabetic control group rats (n=12) and sham group rats (n=6) received 130 mg/kg serum physiologic via orogastric tube. Rats were sacrificed at three weeks. Consequently histologic analysis was done. Results: The mean blood glucoses level 89.8 ± 10.6 on the first day of the study, the mean blood glucoses level 414.7 ± 69.4 72 h after streptozotocin administration (t test; p<0.01). Histological evaluations exposed preserved joint space and were not identified inflammation, increased vascularity and cartilage degeneration with the Haematoxylin-eosin stained. Fibrosis evaluated with Masson’s trichrome stain and type I and type III collagen proportion evaluated with Picrosirius red-stain; no differences were found (Chi-square test; p>0.01). Conclusion: Streptozotocin administration was determined to be an effective experimental model in rats to induce diabetes. Sacrification time not sufficient to reveal the changes in shoulder joint capsule due to the diabetes and the effect of doxycycline. Further studies including larger groups are needed to define the mechanism of the MMPs inhibitors in the shoulder joint in chronic diabetic rats and in the generation of new clinical applications in orthopedics. Level of evidence: Level 1, experimental study.