Abstrakt

Antibacterial Effect of Magnesium Oxide Nanoparticle on Water Contaminated with E.coli

Arsheen Tabassum, Sunita D and M Shailaja Raj

Clean water that is free of toxic chemicals and pathogens is essential to human health. In countries such as India, 80% of the diseases are due to bacterial contamination of drinking water. The World Health Organization recommended that any water intended for drinking should contain fecal and total coliform counts of 0, in any 100 mL sample. When either of these groups of bacteria is encountered in a sample, immediate investigative action should be taken.The antibacterial potential of Magnesium oxide nanoparticle was detected by well diffusion method. Different concentrations of the nanoparticle were analysed by MIC and antibacterial effect. The MIC result reveals that MgO nanoparticle showed maximum inhibition at a concentration of 0.1 g/ml against E.coli. On comparing bactericidal activity it was observed that there was a continuous decrease in the number of colonies with the increase in concentration of MgO nanoparticle. Based on MIC results antibacterial activity of MgO nanoparticle was tested on water sample which was contaminated with E.coli culture. The growth of bacteria is completely inhibited when higher concentration (0.1 g /ml) of MgO nanoparticle was added in to 100 ml of water sample with 5ml of E.coli culture. Therefore from the above studies conducted, it can be inferred that the MgO nanoparticle has significant bactericidal activity, and overall may be more effective in the purification of water sample.