Abstrakt

Determination of Possible Mechanism of Cerebroprotective Action of flavonoid of Dalbergia latifolia against Cerebral Ischemia Reperfusion Induced- Cerebral Infarction in Rats

Sudhir C Kulkarni, Nagarathna PKM, C Vasanthkumar, and N Srisainadh

Dalbergia latifolia is commonly called as “Beete and sitsal”. In the present study the mechanism of cerebroprotective action of methanolic extract of bark of Dalbergia latifolia (DL) was carried out against the global model of ischemia in rats. The animals were pre-treated with DL for a period of 1week (500 mg/kg) p.o. All the inhibitors were given 10 min prior to the occlusion i.p. The animals were anaesthetized with thiopentone sodium (45mg/kg) and infarction was induced by Bilateral Common Carotid Artery Occlusion (BCCAO) for 30 mins with the help of suture threads, the knots were released with the help of slip knots. The animals were allowed to re-perfuse for a period of 48 hrs. Then the animals were sacrificed brain was decapitated. Biochemical estimations for Super oxide dismutase, Catalase, Myeloperoxidase & Malondialdehyde and histological examinations were carried out. The results obtained showed that the Dalbergia latifolia extract is a potent cerebroprotective with a possible mechanism of Cyclooxygenase (COX) pathway inhibitor, as it shown the synergistic activity along with the COX inhibitor.